Spin Probes as Scavengers of Free Radicals in Cells
نویسندگان
چکیده
Spin probes can be used to monitor biological membranes, including the penetration of different molecules into cells. The aim present studies was an investigation endocytosis process two spin labels—2,2,6,6-Tetramethylpiperidine-1-oxyl (TEMPO) and 4-hydroxy-TEMPO (TEMPOL)—into yeast cells a leukemia cell line (HL-60, ATCC CCL-240) by Electron Resonance (ESR). ESR method is helpful for direct detection free radicals. incubation were carried out at 310 K. In contrast, measurements with 240 Spectral differentiation observed; hence, in suspension attached membrane could distinguished. signal changes depended on probe concentration, number, type (healthy/cancerous). Additionally, effect external factors (oxygen vitamin C) decay markers solution established. experimental results prove that (TEMPO TEMPOL) scavenge radicals inside cell. At same time, mechanism interaction determined based low temperatures.
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ژورنال
عنوان ژورنال: Applied sciences
سال: 2022
ISSN: ['2076-3417']
DOI: https://doi.org/10.3390/app12167999